Final Essay Example | Topics and Well Written Essays - 500 words - 11

Final - Essay Example In the initial contemporary period, European encounters were the result of the advancement of change of the western societies, during the revolution into the current capitalist markets and nations to states. The first advancement was experienced in the 15th and 16th centuries, and the focus was the Atlantic basin, which constituted the Atlantic islands, Coastal West Africa, and Central America continent. This also included the Northern Seas, the Oriental Seas and the Asian Seas. The second advancement was in the 18th century, which took place around the Pacific regions. The other expansion was in the 19th century, which took place in the central Africa. All these advancements brought with them new encounters like socialization and movement of people from different parts of the world, which in turn brought about appreciation and development of different cultures or the transcultural world. There was also redistribution of the world resources, which led to imbalance in the world economy as Europeans benefitted more than others (Heinemann 301). These encounters also stimulated the European perception on the nature and community in general, and brought new perspectives of rational enquiry. Also, there were impressive travel accounts and historical writings, which form an important part of history today. Western civilization is viewed as legacy of concepts that exists in Europe and America, which emanated from Greece and Rome. However, the civilization does not belong to any specific place. Different people used historical evidence to promote their own ideas, for instance, Martin Luther’s ideas were based on the Christian teaching in the holy bible. He suggested that, according to the word of God, individuals cannot exist as unmarried without sinning, and it is only in the family where chaste life could hold. Martin Luther encouraged and advocated for removal of girls from the nunneries and covenants that led to sexual repression. Luther

FOIA and Privatization of Government Article Example | Topics and Well Written Essays - 500 words

FOIA and Privatization of Government - Article Example There should a difference in the way of privatizing the traditional governmental services and the private corporation using public funding for non-traditional services because the two are very distinct in the manner of disseminating their duties. The traditional government needs more strict rules in guiding against the events of FWAC because their services affect the entire public and a small flaw in them could lead to the collapse of the whole institution. On the other hand, the private corporations require observance as well but to a smaller extent based on the nature of the population they serve. The examples of FWAC in private corporations and nonprofit corporations that were not subjected to FOIA, but may have been prevented by FOIA include misappropriation of funds by the management, tax evasion, stealing the properties of the firms. Also, giving and taking of bribes, the incorrect use of resources by wasting them and offering favors to the friend and family members. All these FWAC have been prevented by the availability of checks by the FOIA board that has scared those in charge of practicing such vices. The incapacity of the FOIA legislation to force the private corporation to provide information when needed the contracting government agency can mitigate this by setting in the contract the terms that the information regarding the use of public funds should be disclosed to the public. The condition will enable the provision of data concerning the use of the public funds. The private corporations need the support of the government and, therefore, they will be forced to comply and provide the information otherwise they will not have such chances. The issue of concern based on the experience is the style of the provision of the information needed by the public by the FOIA. The fee charged should be reviewed from the set minimum of 15 dollars to as low as 7 dollars.

Culture Of Hepg2 Cells Biology Essay

Culture Of Hepg2 Cells Biology Essay Hep G2 cell line was purchased from American Type Culture Collection (ACTT) (VA, USA). Dulbeccos Modified Eagle Medium (DMEM), 0.5% Trypsin-EDTA 10x, and Penicillin-Streptomycin (PS) were obtained from Invitrogen Corporation (NY, USA). Fetal Bovine Serum (FBS) was gotten from Welgene Inc. (Daegu, South Korea). Fatty acids (Palmitic, Oleic and Dedocanoic acid), Dimethyl sulfoxide (DMSO) and Tween 20 came from Sigma (MO, USA). Bovine serum albumin (BSA) was from Santa Cruz Biotechnology (CA, USA). MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) was purchased from Molecular Probes (Oregon, USA). LDH assay (Lactate dehydrogenase assay) was from ROCHE (Mannhein, Germany). [emailprotected]/503 and Carboxyl-H2DCFDA were purchased from Invitrogen Corporation (Oregon, USA). Nile red was from Fluka (MO, USA). Triglyceride Quantification Kit and ATP Colorimetric/Flourometric Assay Kit were purchased from BioVion Inc. (CA, USA). Annexin V Floustaining kit was from Roche (IN, USA). Phosphate buffered saline was made up of chemicals at pH 7.4, including 11.7g NaCl, 5.5g Na2HPO4-7H2O, and 1.35g NaH2PO4. All other chemicals met in standard grade of analysis. Culture of HepG2 cells HepG2 cells were cultured in Dulbeccos modified Eagles medium, containing 10% (v/v) fetal bovine serum and 1% (v/v) Penicillin-Streptomycin under 5 % CO2, 95 % humidity at 37 °C. The cells were subcultured by using 0.5% Trypsin-EDTA 1x (Invitrogen Corporation, NY, USA) for detachment and seeded at proper cell number in all experiments. Fatty acid treatment When 80 % confluency of HepG2 was reached, it was treated with various concentrations of the fatty acids (0 mM, 0.1 mM, 0.2 mM, 0.3 mM, 0.5 mM, 0.7 mM and 1.0 mM) for 24 h. The stock solution of fatty acids was prepared at 100 mM by dissolving in DMSO and stored at -200C. The stocks were diluted in DMEM media containing a constant ratio of fatty acid bound bovine serum albumin at 2 to 1 to obtain working solution in all experiments. Cytotoxicity assay Cytotoxicity was based on the measurement of cytoplasmic enzyme activity by using cytotoxicity detection kit (ROCHE, Mannhein, Germany). The cytoplasmic enzyme was released from damaged cells that its enzyme activity expresses to the proportion of toxiced-cell. Lactate dehydrogenase (LDH) presents in all cells which is a stable cytoplasmic enzyme. When the membrane integrity of the cells is damaged, it is quickly released into the media. In this assay, NAD+ is reduced to NADH/H+ during conversion of lactate to pyruvate by the LDH-catalyzed. After that, H/H+ from NADH/H+ was transferred by the catalyst (diaphorase) to the tetrazolium salt (yellow) which was reduced to formazan (red). To conduct the assay, the culture supernatant is collected cell-free after desire exposure time (24 h). The reaction mixture from the kit was then applied in the samples. The absorption of the formazan dye formed was measured at 490 nm on an ELISA reader (VERSARMAX, Molecular Divices., CA, USA). Cell viability Cell viability was measured based on measurement absorption of a water-insoluble purple formazan which was reduced from a yellow water-soluble tetrazolium salt in live cells. Briefly, the cells were treated with MTT (5 mg/ml) in DMEM at 37 0C for 1.5 h. Then, the media were removed, and DMSO was added to dissolve the furmazan crystals. After gently pipetting, the absorbency was measured at 570 nm using an ELISA reader (VERSARMAX, Molecular Divices., CA, USA). The estimation of cell viability was calculated by comparing between the spectra value of treated and untreated cells. Quantification of triglyceride Triglyceride content (TG) was determined according to an enzymic method (BioVion Inc, CA, USA). In this method, glycerol is a product by TG-catalyzed which reacts with the probe to generate coloration measured on spectrophotometry at 570 nm. In briefly, the cells were washed twice times with cold PBS, then homogenized in 5% Triton-X100 solution. After slowly heating at 80-100 °C for 5 min, the samples were centrifuged at 12000 rpm for 5 min. The supernatant collected from removing insoluble materials was added 2 ÃŽ ¼l of lipase, mixed well and incubated for 20 min at room temperature. Finally, 50 ÃŽ ¼l of the reaction mix was putted in each sample for 45 min of incubation, protected from light. The value of triglyceride content was quantified based on triglyceride standard curve that was constructed with different concentrations of TG (0, 20, 40, 60, 80, and 100 nmol/ml). Measurement of reactive oxygen species (ROS) generation The measurement of ROS production within cells was carried out by using 2†²,7†²-Dichlorohydrofluorescein diacetate (Carboxyl-H2DCFDA; Invitrogen Corporation, Oregon, USA) which is combined into fluorescent products in the presence of H2O2 and other ROS molecules and esterases (Zhenyuan Song et al, 2007). After the cells were overloaded with 1.0 mM fatty acids, 10 mM final concentration of Carboxyl-H2DCFDA was added in the media without FBS at 370C in darkness for 30 min. Then, the cells were washed twice times with warmed PBS and lysed in 200 Ã‚ ­l RIPA buffer (PIERCE, IL, USA). The lysed-cells were centrifuged at 12000 rpm for 5 min. The supernatants were conveyed to a 96-well back plate which were excited at 485 nm and emitted at 530 nm for the Carboxyl-H2DCFDA fluorescence on Fluorometer (VICTOR2, Perkin Elmer., MA, USA). Trilyceride staining on Confocal Bodipy @493/503 (Invitrogen, Oregon, USA) was used to capture TG fluorescence on Confocal microscopy. In this experiment, the cells were prepared as above. Before the dyes treatment, the cells were washed with PBS twice times. Bodipy @493/503 was then added at 1.0  Ã‚ ­M, and 15 min of incubation at 370C after the cells were rinsed with PBS again. Zeiss LSM Image Brown software (LSM 510 meta, Carl Zeiss., Jena, Germany) was handled to take TG image at excitation of 488 and emission of BP 505-530 nm. ROS staining on Confocal ROS generation in HepG2 was stained by using Carboxyl-H2DCFDA. In this experiment, the cells were prepared as above. Before the dyes treatment, the cells were washed with warmed PBS twice times. The carboxyl-H2DCFDA was applied at 10 mM final concentration in Serum free media (DMEM without FBS), and incubated for 30 min at 370C, protected from the light. After that, the cells were rinsed with warmed PBS again. Zeiss LSM Image Brown software (LSM 510 meta, Carl Zeiss., Jena, Germany) was handled to take ROS image at excitation of 488 nm and emission of LP 530 nm. Detection of cell death and trilyceride accumulation by Confocal HepG2 seeded in the 24-well plate and treated with final concentration of fatty acids to 1.0 mM for 24 h. After the incubation time, the cells were washed twice times with PBS. Then, Bodipy @493/503 (Invitrogen, Oregon, USA) was dissolved in PBS at 5  Ã‚ ­g/ml which was added into each well. This process was kept in darkness for 15 min at 370C. After that, the Bodipy solution was removed and the cells were then washed by Binding buffer from Annexin V Floustaining kit (Roche, IN, USA). Finally, the cells were incubated in 100  Ã‚ ­l/ml of Propidium iodide (PI) for 10 min in darkness. Exposition of TG accumulation and apoptosis was observed at excitation of 488 and 543 nm, and emission of BP 505-530 and LP 650 nm on Carl Zeiss Confocal Microscopy (LSM 510 meta, Carl Zeiss., Jena, Germany), respectively. Data analysis All results were expressed as mean of repeated three or four values  ± SEM. The difference between groups was identified by using t.test. p < 0.05 was considered statistical significant.

The Life and Times of Rosie the Riveter Essay -- American History

The film titled, â€Å"The Life and Times of Rosie the Riveter†, looks at the roles of women during and after World War II within the U.S. The film interviews five women who had experienced the World War II effects in the U.S, two who were Caucasian and three who were African American. These five women, who were among the millions of women recruited into skilled male-oriented jobs during World War II, shared insight into how women were treated, viewed and mainly controlled. Along with the interviews are clips from U.S. government propaganda films, news reports from the media, March of Time films, and newspaper stories, all depicting how women are to take "the men’s" places to keep up with industrial production, while reassured that their duties were fulfilling the patriotic and feminine role. After the war the government and media had changed their message as women were to resume the role of the housewife, maid and mother to stay out of the way of returning soldiers. Thus the patriotic and feminine role was nothing but a mystified tactic the government used to maintain the American economic structure during the world war period. It is the contention of this paper to explore how several groups of women were treated as mindless individuals that could be controlled and disposed of through the government arranging social institutions, media manipulation and propaganda, and assumptions behind women’s tendencies which forced â€Å"Rosie the Riveter† to become a male dominated concept. Since the war began women were led to believe that they were the ones who had to be the patriotic sacrifice until the men came home from war. The film reveals how the government used the media to alternately urge women to give up such elements of their feminin... ...owards more love stories. Essentially more forms of propaganda ensued to let women know what they should be doing. More domestic jobs became available such as being a maid, restaurant work, dishwashing and cleaning. However women who worked war jobs wanted their own maids now so they could pursue their own dreams. They felt inspired and accomplished. Lola Wiexl mentioned that although skills within the workforce were easily learned, within the household traditions still persisted. Lola herself said she'd go home cook, clean and do the laundry while her brother laid on the couch. She didn't question it before but she was angry about it for years after her war time experience. Thus patriarchal hegemonies still existed after the war and were perpetuated by the government and media as much as possible to solicit women who participated in activities outside of the home.

Character Sketch Example: Mirror Image

The story â€Å"Mirror Image† by Lena Coakley is about the character Alice trying to find her true self in the midst of an identity crisis. The character’s struggle was caused when she and her dad had an accident where she made it but her dad didn’t. But the thing is, her body didn’t quite make it too. So they had to do a brain transplant. Now Alice is considered as a new person by her sister but she is in denial with it. Her twin sister Jenny is also quite upset and feels she is but a stranger to their home. Having a new body, she described her appearance in the story. On her new body, she seems to be slim and more mature than her old body. It is proved when she said there were no cellulites on her thighs which are fats found in thighs and buttocks. Also, she said her body weighed much more than her old body meaning her new body is more mature . Another trait indicated in the story was she had clean and pretty face. On the story, Alice said that no one told her that she was ugly and her face never had zits on it. Lastly she had big brown eyes. In the hospital when she’s in bed and got her body, when she looked into the mirror, she saw an unfamiliar face, her face with big brown eyes. Alice can be described as an adaptive person. She learned to adapt to her new body and to her new friends, new environment. Alice is also a proud person. She keeps boasting about her new features compared to old body. Alice is also confident. Since she got her body replaced she decided to join cheerleading. And she had the confidence that Alice and her family will get through this struggle of hers. In the story, the character focused on finding herself. Her motivation was to prove what’s inside that body of hers is the same Alice they know and used to be with. Evidence is that her sister won’t let her read her diary for a reason that Jenny is no longer comfortable being with her and looks at her as a stranger to their house. Also, it is on the part where she tasted the cake and her taste buds didn’t like it. She wants to prove that the chocolate with mocha cream cake that her mother made for her was still her favourite cake even after the body change. Lastly, Alice wants to show that what Mr. Jarred saw and talked to was not her daughter Gail, but a different person, her accent, the way she walks and even when he looked into her eyes. If brain transplant were ever possible, anyone may be in a position like Alice where she had to go find her true self lost in the midst of an identity crisis. It may be hard but the character was able to adapt to her new body, following the consequences that her sister sees her as a stranger and unsure about her having a new body.

A and P: Movie Review

Gallant After watching the film A&P In comparison to the discussion In the classroom, a lot of the topics are current trends and Issues In the 20th century. There can be much discussion about many things in the film. After watching the film however rather than from the reading and discussion in class, I pictured things differently in my own mind. Upon first trying to relate it to the sass's, I actually thought the film was pretty odder and could be possible in today's times, depending on a store and its' management, whether an independently owned little store or a big box grocery store. The main character, Sammy, who displays an 18 or 19 year old male teenagers' feelings are very much alike as all boys during the adolescence years. John Update himself reported In his Interview of when he was young and married and 29 years of age that he could relate to these types of feelings and fantasy dreams which prompted him to write the story.I could not envision however In the sass's of actual ly seeing any young girls in bathing suits that would be daring enough to walk in a store dressed in such a way. Personally, I do not recall seeing any young teenage girls do this during those times. I did grow up in the sass's. I recall most young women being more demure and even seeing something such as this might result in a police arrest possibly. Perhaps I am being naive as I was a young child during this period but don't recall families allowing their young girls to behave in such a way.Sammy appears to be bold In that time period In my opinion of how he chose to Just quit his Job because he TLD believe the same beliefs as his manager, Mr.. Lange. While growing up In the sissy, many children and adults alike, from what I saw and remember were taught to work hard and success would then follow. Many of the male adult family relatives were away serving in the Navy or Army and those at home, especially the women, had chores at home, like laundry and cooking, were working hard stil l because they had larger families to feed.My grandmother who had 13 or 14 hillier had also worked part time in a retail chain called J. J. Newbury. Our family would never have heard of a teenage boy being able to quit his job Just because he didn't agree with his manager who was his elder. Teenagers growing up in the sass's whom I was exposed to had to respect their elders and do as adults around them expected and work hard for their families with chores to do along with school work in order to receive allowances from the family In return for the hard work. The Job was Important If a teenager had one to share the wages with their family.Regarding consumers as a society of pigs, cows, and sheep, and an older woman's' attitude compared to a witch that should be burned in Salem was never even a be rebelling to soon becoming an adult in the near future and possibly could be one of these people. He is suppressing some self-discovery and trying to figure out where he fits in a society an d perhaps wondering if he belongs working in a grocery store for the rest of his life or if he really should be having fun like the young girls laughing and running about in the store.

Grant Wood, American Gothic Painter

Grant Wood, American Gothic Painter Grant Wood (1891 -1942) is one of the best-known and most revered American artists of the 20th century. His American Gothic painting is iconic. Some critics derided his regionalist art as influenced by pernicious political theories. Others saw hints of sly camp humor impacted by Woods closeted homosexuality. Fast Facts: Grant Wood Occupation: PainterStyle: RegionalismBorn: February 13, 1891 in Anamosa, IowaDied: February 12, 1942 in Iowa City, IowaSpouse: Sara Maxon (m. 1935-1938)Selected Works: American Gothic (1930), Midnight Ride of Paul Revere (1931), Parson Weems Fable (1939)Notable Quote: All the good ideas I ever had came to me while I was milking a cow. Early Life and Career Born in rural Iowa, Grant Wood spent much of his childhood on a farm. His father died suddenly in 1901 when Grant was ten years old. Following the death, his mother moved their family to the small nearby city of Cedar Rapids. Along with his older brother, Grant Wood took odd jobs to help provide financial support for their family. Wood showed an interest in drawing and painting while attending Cedar Rapids public schools. He submitted his work to a national competition in 1905 and won third place. The success cemented his determination to be a professional artist. Grant Woods boyhood home in Cedar Rapids, Iowa. Bill Whittaker / Wikimedia Commons / Creative Commons 3.0 While in high school, Grant Wood began designing stage sets with fellow artist Marvin Cone and began volunteering at the Cedar Rapids Art Association, which later became the Cedar Rapids Museum of Art. Following high school graduation, Wood took a summer course at the Minneapolis School of Design and Handicraft in Minnesota. He also took art classes at the University of Iowa. In 1913, Grant Wood moved to Chicago, making jewelry to support himself and his night classes at the Art Institute of Chicago. Following the failure of his jewelry business, Wood returned to Cedar Rapids in 1916 and worked as a home builder and decorator to support his mother and his youngest sister, Nan. Rise to Prominence After World War I ended in 1919, Grant Wood took a position teaching art at a local Cedar Rapids middle school. The new income helped finance a trip to Europe in 1920 to study European art. In 1925, Wood left his teaching position to focus on art full time. Following a third trip to Paris in 1926, he decided to focus on the common elements of life in Iowa in his art, making him a regionalist artist. Residents of Cedar Rapids embraced the young artist and offered jobs designing stained glass windows, executing commissioned portraits, and creating home interiors. In the wake of national recognition for his paintings, Grant Wood helped form the Stone City Art Colony in 1932 with gallery director Edward Rowan. It was a group of artists who resided near Cedar Rapids in a village of whitewashed, tidy wagons. The artists also taught classes at nearby Coe College. Midnight Ride of Paul Revere (1931). Francis G. Mayer / Getty Images American Gothic In 1930, Grant Wood submitted his painting American Gothic to a show at the Art Institute of Chicago. It depicts, presumably, a farming couple, either married or a father and daughter, standing in front of their frame house with a large gothic window. The models for the couple were Grant Woods dentist and his younger sister, Nan. The Chicago Evening Post published an image of American Gothic two days before the show, and it became practically an overnight sensation. Newspapers across the country reproduced the image, and the Art Institute of Chicago bought the painting for their permanent collection. Initially, many Iowans criticized the work thinking that Grant Wood depicted them as grim-faced Puritans. However, some saw it as satire, and Wood insisted that it represented his appreciation for Iowa. American Gothic (1930). GraphicaArtis / Getty Images American Gothic remains one of the most iconic American paintings of the 20th century. Countless parodies from Gordon Parks stunning 1942 photo American Gothic, Washington, D.C. to the closing image of the opening credits for the 1960s TV show Green Acres are a testament to the enduring power of the portrait. Later Career Grant Wood painted most of his key works in the 1930s, including 1931s Midnight Ride of Paul Revere,- a theatrically-lit depiction of Henry Wadsworth Longfellows legendary poem- , and 1939s unique take on the George Washington cherry tree legend in Parson Weems Fable. During the period, he also taught art at the University of Iowa. By the end of the decade, he was one of the most famous American artists. Parson Weems Fable (1939). Amon Carter Museum / Wikimedia Commons / Public domain Unfortunately, the final three years of Grant Woods life and career were rife with frustration and controversy. His ill-considered marriage, according to his friends, ended in the late 1930s. Lester Longman, a devotee of European-led avant-garde modern art, became the chair of the art department at the University of Iowa. After clashes with Wood and public efforts to discredit him, the universitys most famous artist left his position in 1941. Later investigations discovered that rumors of homosexuality also drove some of the efforts to remove him from the university faculty. In 1941, just as it seemed that some of the controversies were settling down, Grant Wood received a diagnosis of pancreatic cancer. He died a few months later in February 1942. Legacy For many casual observers of art, Grant Wood remains one of the most popular and revered of 20th-century American artists. Along with Thomas Hart Benton, Wood is one of the most prominent of American regionalist painters. However, the controversies that began at the University of Iowa have raised questions about his reputation since. Some critics dismissed regionalism as inspired by fascist and communist principles. Daughters of Revolution (1932). Francis G. Mayer / Getty Images Art historians also continue to reevaluate Grant Woods art in the light of his closeted homosexuality. Some see the satire and double meanings in his work as part of the camp humor sensibility in gay culture. Sources Evans, R. Tripp. Grant Wood: A Life. Knopf, 2010.Haskell, Barbara. Grant Wood: American Gothic and Other Fables. Whitney Museum of American Art, 2018.